BioLink™ GreenLink™ Blue qPCR Mastermix is a next-generation 2X Master-mix for use in real-time PCR (qPCR) applications offering a broad dynamic range with industry-leading sensitivity, specificity, speed, and reproducibility. It contains an inert, blue tracer dye to facilitate accurate pipetting, which has no effect on accurate quantitation.
GreenLink™ Blue qPCR Mastermix utilizes the proprietary GreenLink™ fluorophore, a highly-sensitive intercalating dye-based detection chemistry that exhibits minimal PCR inhibition and greater fluorescence than SYBRⓇ Green I, generating earlier Ct values. Data may be acquired on the SYBR/FAM channel (520 nm) with no change in instrument settings. Melt curve analysis may be performed following qPCR to ensure specific product formation.
Our antibody-mediated LinkTaq™ hot-start polymerase demonstrates high-specificity with industry-leading processivity, and is compatible with both standard and fast reaction speeds. Our fast protocol allows for 40 cycles in under 20 minutes, maximizing laboratory throughput.
Check our instrument compatibility chart to determine which of our mastermixes is recommended for your instrument.