BioLink™ One-Step kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR mastermix and reverse transcriptase.
Our optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase, which enables robust first strand cDNA synthesis. BioLink™ Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial denaturation step at 95°C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.
Two versions of our One-Step qPCR kits are available:
BioLink™ GreenLink™ One-Step kits incorporate our proprietary GreenLink™ intercalating dye, which exhibits higher fluorescence and lower PCR inhibition than other popular dyes, such as SYBR® Green.
BioLink™ Probe One-Step kits are optimized for use with hydrolysis probes, such as popular TaqMan™, Scorpions®, and molecular beacon probes.
Both One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed, and reproducibility for a broad range of samples and targets. Our mastermixes are available with different levels of ROX reference dye for compatibility with all qPCR instrumentation.