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Home / PCR and qPCR / PCR and qPCR Reagents / LinkTaq™ HS Taq Polymerase (Hot Start)

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LinkTaq™ HS Taq Polymerase (Hot Start)

LinkTaq™ HS

    • Industry leading specificity, yield, and speed
    • Antibody-mediated hot-start
    • Perfect for low copy number applications, multiplex, and direct PCR
    • Fast mode – 40 cycles in <33 minutes
    • Suitable for the amplification of complex, GC- and AT-rich templates
    • Available with inert red dye for convenient loading onto an agarose gel

$137.00 – $1,525.00

16-1000-HS-500   $183.00

— OR —

16-1000-HS-1000   $316.00

— OR —

16-1000-HS-6000   $1,525.00

— OR —

16-1001-HS-200   $137.00

— OR —

16-1001-HS-1000   $560.00

— OR —

16-1001-HS-200R   $137.00

— OR —

16-1001-HS-1000R   $560.00

— OR —

Product price
Additional options total:
Order total:
SKU: 16-1000-HS Series

Category: PCR and qPCR Reagents
  • Description
  • Additional information
  • specifications
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Description

BioLink™ LinkTaq™ Hot-Start (HS) is our most popular polymerase for low copy number, multiplex, direct, and high-throughput PCR applications. 

LinkTaq™ HS offers industry leading sensitivity, specificity, and speed thanks to the boosted processivity of our LinkTaq™ HS polymerase.

Our R&D team performed site-directed mutagenesis on the active site of Taq polymerase, which increased its processivity significantly and generated LinkTaq™ HS.  With improved processivity, LinkTaq™ HS is a fast-mode compatible enzyme and offers 15 second/kb extension times and a 40-cycle protocol in less than 33 minutes.

With improved processivity also comes improved enzyme efficiency and in turn, greater yield and sensitivity.  LinkTaq™ HS is, therefore, an excellent choice for low copy number applications.

LinkTaq™ HS offers robust and reproducible performance even with GC and AT-rich templates or crude samples and generates products up to 6kb.

A monoclonal antibody potently inhibits the activity of LinkTaq™ HS prior to initial denaturation at 95°C, allowing for reaction setup at room temperature and greatly reducing the potential for nonspecific amplification and primer-dimer formation.

LinkTaq™ HS is available in two formats: a 2X Mastermix – just add template and primers, or in a 2-tube format containing a 5X reaction buffer (without dNTPs) and LinkTaq™ HS Polymerase (5u/uL).  The Mastermix is available with an optional inert, red gel-loading dye, which allows for the direct loading of PCR product into gels.  

Weight1 lbs
Dimensions5 × 7 × 8 in

specifications

Polymerase:

LinkTaq™ HS

Hot-Start?:

Yes, antibody-mediated

Maximum Product Size:

6kb

Product Overhang:

3'-A

Reaction Speed:

Fast and Standard

Exonuclease Activity:

5'-3'

Storage:

12 months at -20°C (or 4°C for up to 1 month)

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biolink laboratories llc.

Web: www.BioLinkLaboratories.com

Ph: 202.848.3463    EM: info@biolinklab.com