BioLink Agarose LE is a highly purified agarose, designed for a variety of molecular biology applications. It is widely used for nucleic acid electrophoresis (analytical and preparative) for various sizes (150 bp to 25 kbp), protein electrophoresis (including radial immunodiffusion) and various blotting protocols. It is easily soluble, free of nucleases and easy to use. It is highly transparent (forms a clear, colorless solution at 1g:100mL H2O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.
It’s low EEO (≤ 0.13-mr ) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.
Formulated for high gel strength and integrity, BioLink Agarose provides exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, even if a denaturing agent has been added.