Description

Optimized DNA transfection efficiency on hard-to-transfect cells

jetOPTIMUS^® is an innovative cationic nanotechnology developed to improve DNA transfection efficiency in easy- and difficult-to-transfect cells used as in vitro cell culture models. Tested on various primary cells and cell lines, jetOPTIMUS^® proved its superiority by reaching higher transfection efficiencies and gene expression than main competitors.

Advanced nanotechnology: Superior gene expression using less material!

jetOPTIMUS^® has been developed to increase transfection efficiency into a wide range of cell types, while keeping the amount of DNA as low as possible, and reducing the volume of reagent required compared to main competitors. Consequently, scientists can increase the number of transfections and reduce the cost per reaction by up to seven-fold (table 1).

Excellent cell viability and morphology

Reaching high transfection efficiencies is often at the expense of cellular integrity, with adverse effects on cell cycle, metabolism and signaling pathways. At Polyplus-transfection, we make no compromise on cell integrity: jetOPTIMUS^® transfection reagent is gentle on cells, allowing generation of biologically relevant data from gene expression studies.

Optimized transfection protocol

jetOPTIMUS^® is a ready-to-use transfection reagent provided with its own complexation buffer (jetOPTIMUS^® Buffer). The protocol is optimized for simplicity of use (all plate sizes), culture medium compatibility (antibiotics, serum) and cost-effectiveness (lowest amount of DNA and volume of reagent).