PREVENT – DETECT – ELIMINATE
Protect your cells from the harmful effects of mycoplasma
Mycoplasma is a common contaminant of cell cultures and bio-pharmaceutical production. Research shows that approximately 30 to 85% of cell cultures are infected with mycoplasma. Mycoplasma contamination is likely to negatively impact cell growth, synthesis of nucleic acids, and protein expression.
Signs of mycoplasma contamination include:
– Change in cell morphology
– Change in growth pattern
– Decrease in cell activity
– Poor transfection
– Observation of black particles in culture media
– Observation of black holes at cell surface
The Myco-X™ Mycoplasma PCR Detection Kit offers an all-in-one solution to mycoplasma testing. 48 pre-aliquoted PCR reactions are provided in a supermix containing a Hot-Start Taq Polymerase, dNTPs, mycoplasma primers, internal control primers and 8-MOP. The kit also includes control DNA and DNase/RNase free DI water. All that’s needed for testing is template DNA. Our protocol may be completed in under 3 hours with sensitivity down to 10 CFU/mL.