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Home / Transfection Reagents / Life Science Research / in vivo-jetPEI® in vivo DNA, si/miRNA transfection

in vivo-jetPEI® in vivo DNA, si/miRNA transfection

    • Polyvalent: in vivo delivery of DNA, si/sh/miRNA in any animal model
    • Easy to use: two-step protocol
    • Renowned: Most advanced in vivo delivery technology with over 700 peer-reviewed publications
    • Successful: Used from fundamental research to Human clinical trials

$662.00 – $2,328.00

20-101000040   $662.00
20-101000030   $2,328.00
SKU: 20-201 Series

Category: Life Science Research
  • Description
  • specifications
  • Reviews (0)

Description

Polyvalent: in vivo delivery of DNA, si/sh/miRNA nucleic acid in any animal model including mice

The easiness of use and versatility of in vivo-jetPEI® allows scientists to perform gene function studies by overexpressing or downregulating a gene in any organ/tissue of interest. in vivo-jetPEI® is polyvalent: it is suitable for the delivery of nucleic acid(plasmid DNA, siRNA, shRNA, miRNA and oligonucleotides) in any animal model (mice, rat, guinea pig, dog, rabbit, monkey, etc…). Numerous Scientific publications demonstrate the successful delivery of each type of nucleic acid in different animal model.

FIND MORE INFORMATION ON OUR ONLINE IN VIVO TRANSFECTION DATABASE

in vivo-jetPEI® has already been used to target a wide range of organs by systemic and local injection routes. Local administration routes include subcutaneous tumor, intracerebral or intra-articular injections and topical application. The injection route largely determines the targeted organs. For example, upon intravenous injection, in vivo-jetPEI®-mediated DNA delivery leads to gene expression mainly in the lung but also in the liver, pancreas, spleen, kidney, heart, bladder and artery. Conversely, upon intraperitoneal injection, the gene of interest will be expressed in the ovary, pancreas, diaphragm, uterus and stomach (Fig. 1). To achieve organ specific gene expression, cell-specific promoters can be combined with the choice of a local injection route to restrict gene expression to an organ/tissue. Guidelines for systemic and most local injection routes are available.

<em>in vivo</em>-jetPEI - Intravenous injection & intraperitoneal injection in mice
Fig. 1: Organs targeted following systemic nucleic acid delivery using in vivo-jetPEI® in mice. Complexes were formed using 40 μg or 100 μg of luciferase expressing plasmid and in vivo-jetPEI® at an N/P ratio of 8, in 200 μl or 1 ml of 5% glucose and injected either through retro-orbital sinus (IV) or intraperitoneally (IP), respectively. 24 hours after injection, different organs were extracted and luciferase expression was measured or live imaging was performed using IVIS system (Perkin Elmer).

Easy-to-use: two-step protocol

 in vivo-jetPEI® is the reagent of choice to deliver DNA, si/sh/miRNA nucleic acid using most systemic and local injection routes. The protocol is easy to use and similar to a classical in vitro transfection: the nucleic acid and in vivo-jetPEI® reagent are mixed and directly injected into the animal model (Fig. 2).

<em>in vivo</em>-jetPEI - easy 2-step protocol
Fig 2: in vivo-jetPEI® protocol in mice. This two-step protocol is compatible with direct injection of in vivo-jetPEI®/nucleic acid nanoparticles though any systemic or local administration routes.

Our delivery experts are available to adapt your protocol to your animal model and send you the relevant literature.

CONTACT TECHNICAL SUPPORT

Renowned: Most advanced in vivo delivery technology for cancer research, immunization and vaccination

in vivo-jetPEI® is a powerful polymer-based reagent with unique properties. In the provided complexation solution, in vivo-jetPEI® condenses any nucleic acid into stable nanoparticles of ca. 50 nm diameter (Fig 1). These nanoparticles are sufficiently small to efficiently diffuse within tissues and enter cells by endocytosis, while protecting naked nucleic acids from degradation. At the cellular level, in vivo-jetPEI facilitates both endosomal escape using the proton sponge mechanism (Akinc et al. (2005), J Gene Med 7: 657), and crossing of the nuclear membrane (Brunner et al. (2002), Mol Ther 5: 80).

<em>in vivo</em>-jetPEI - complex size
Fig. 3 in vivo-jetPEI® forms small spherical particles with plasmid DNA. in vivo-jetPEI®/DNA complexes are prepared in 5% glucose solution at N/P ratio of 10. Complexes were added on a carbon covered grid and stained with uranyl acetate. Observation was carried out under a TEM. Bar is 100 nm. Complexes produced in glucose solution are discrete spheres having a mean size of 50 +/- 30 nm (Courtesy J-S Remy, Laboratoire Chimie Génétique, CNRS UMR 7514, Illkirch, France).

Successful: Used from fundamental research to Human clinical trials

Due to its reliability, in vivo-jetPEI® has been selected as a delivery vector for several drug development programs due to its safety and delivery efficiency. There are currently several ongoing clinical trials for cancer therapies, vaccination and immunization using higher quality grade GMP in vivo-jetPEI®.

specifications

Brand:

PolyPlus-transfectionⓇ

Molecule Delivered:

DNA, siRNA, miRNA, oligonucleotides

Applications:

in vivo functional studies (overexpression, knock-down, CRISPR genome editing), Cancer Research, Vaccination/Immunization

Targeted Organs:

All Organs

Injection Routes:

Various systemic and local administration routes

Number of Transfections:

100 µl of in vivo-jetPEI® delivery reagent is sufficient to perform 15 to 25 intravenous injections in mice

Storage:

-20 ± 5 °C, for at least 12 months

Provided with:

10% glucose solution

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Protocols and Supporting Documentation

in vivo-jetPEI Protocol

in vivo-jetPEI Guidelines for Mice

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