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Home / PCR and qPCR / PCR and qPCR Reagents / BioLink™ PrimeChoice™ cDNA Synthesis Kit

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BioLink™ PrimeChoice™ cDNA Synthesis Kit

BioLink™ PrimeChoice™ cDNA Synthesis Kit

    • Generates cDNA amplicons up to 9kb in length
    • Optimized for a wide range of input RNA: 2µg-10pg total RNA
    • Includes random hexamers & oligo (dT) primers in separate tubes for assay flexibility

$107.94 – $435.18

16-2110-50   $107.94
16-2110-200   $435.18
SKU: 16-2110 Series

Category: PCR and qPCR Reagents
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  • specifications
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Description

The BioLink™ PrimeChoice™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first-strand cDNA from total RNA or mRNA templates. The MMLV-derived high-capacity reverse transcriptase is modified for improved sensitivity with challenging templates, and greater thermostability. The enzyme, which is capable of synthesizing cDNA up to 9kb, is blended with a potent RNase inhibitor for greater stability and performance with a wide range of RNA sources.

specifications

Kit format:

4-tubes: 5X reaction buffer, high-capacity RT enzyme(100u/uL), random hexamer primers (20µM), and oligo (dT) (20µM)

Protocol duration:

70-80 minutes

RNase H+ activity:

Reduced

Product size:

Up to 9kb

Optimal reaction temperature:

42°C (45°C for high-GC content)

Loading capacity:

2 μg to 10 pg RNA

Storage:

-20°C for up to 12 months

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biolink laboratories llc.

Web: www.BioLinkLaboratories.com

Ph: 202.848.3463    EM: [email protected]