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BioLink™ PrimeChoice™ cDNA Synthesis Kit
$107.94 – $435.18
The BioLink™ PrimeChoice™ cDNA Synthesis Kit is a complete and flexible system for efficient synthesis of first-strand cDNA from total RNA or mRNA templates. The MMLV-derived high-capacity reverse transcriptase is modified for improved sensitivity with challenging templates, and greater thermostability. The enzyme, which is capable of synthesizing cDNA up to 9kb, is blended with a potent RNase inhibitor for greater stability and performance with a wide range of RNA sources.
Kit format:
4-tubes: 5X reaction buffer, high-capacity RT enzyme(100u/uL), random hexamer primers (20µM), and oligo (dT) (20µM)
Protocol duration:
70-80 minutes
RNase H+ activity:
Reduced
Product size:
Up to 9kb
Optimal reaction temperature:
42°C (45°C for high-GC content)
Loading capacity:
2 μg to 10 pg RNA
Storage:
-20°C for up to 12 months
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