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Home / Transfection Reagents / Life Science Research / jetPRIME® Transfection Reagent for DNA/siRNA

jetPRIME® Transfection Reagent for DNA/siRNA

    • High DNA transfection efficiency
    • Low amounts of nucleic acid
    • Superior cell viability
    • ONE reagent for DNA and/or siRNA transfections

$405.00 – $3,148.00

20-101000015   $405.00
20-101000046   $688.00
20-101000001   $3,148.00
SKU: 20-114 Series

Category: Life Science Research
  • Description
  • Additional information
  • specifications
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Description

Superior transfection efficiency

jetPRIME® is a powerful transfection reagent for day-to-day experiments. It leads to unusually high percentage of transfected adherent cell lines of various origins as well as primary cells.

Superior transfection efficiencies ranging between 70 and 90% were obtained with jetPRIME® reagent versus the top competitor’s reagent for several commonly used cell lines (Fig.1 & Fig.2).

jetPRIME - Comparison Lipofectamine 2000
Fig. 1: Comparative transfection efficiency of jetPRIME versus its main competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection in 24-well plates. Conditions were used according to the manufacturer’s recommendation for both Lipofectamine® 2000 and for jetPRIME®.
jetPRIME - Comparison Lipofectamine 3000
Fig. 2: Comparative transfection efficiency of jetPRIME® versus Lipofectamine® 3000. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection in 96-well plates or 24-well plates. Conditions were used according to the manufacturer’s recommendation for lipofectamine® 3000 and for jetPRIME®.

Cost-effective: less DNA and less reagent

jetPRIME® is a powerful in vitro transfection reagent that requires a small amount of reagent and plasmid DNA, making its use very cost-effective (Table 1).

jetPRIME - table competitors
Table 1: Recommended conditions to use. Amounts of DNA and reagent (jetPRIME® and competitors) per well in 6-well plate for transfection according to manufacturers’ recommendations.

In addition to reducing costs, using less DNA also minimizes adverse cytotoxic effects triggered by transfection. Hence, jetPRIME® is the reagent of choice for high transfection efficiency with excellent cell viability.

Better cell viability

jetPRIME® is extremely gentle on cells during transfection leading to increased cell viability and improved transfection results. Cells transfected with jetPRIME® are healthy, while major cytotoxicity is observed with competitors (Fig.3).

jetPRIME - transfected cells compared to reagents L2K and L3K
Fig. 3: Cell viability 24h after transfection. Phase contrast microscopy of HeLa cells 24 h after transfections performed according to the manufacturer’s recommendations for each reagent.

Easy to use protocol

jetPRIME® is an easy-to-use transfection reagent (Fig.4):

  • Fast and easy to scale up and down
  • Compatible with serum and antibiotics
jetPRIME - protocol
Fig. 4: jetPRIME® protocol. Convenient protocol for DNA, siRNA and co-transfection of DNA and siRNA.

Weight 6 lbs
Dimensions 10 × 9 × 8 in

specifications

Brand:

PolyPlus-transfectionⓇ

Molecule Delivered:

DNA, siRNA, DNA & siRNA

Cell Types:

Adherent cell lines grown in presence of serum

Number of Transfections (/1.5ml)

1500 transfections in 24-well plates

375 transfections in 6-well plates

Storage:

5°C (+/-3°C) for 1 year

Provided With:

jetPRIME Buffer

Applications::

Plasmid transfection, Genome editing, Virus production, siRNA transfection, Co-delivery of different nucleic acids

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Protocols and Supporting Documents

jetPRIME Flyer

jetPRIME Short Protocol

jetPRIME Protocol

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biolink laboratories llc.

Web: www.BioLinkLaboratories.com

Ph: 202.848.3463    EM: [email protected]