Description

BrilliantDye™ Terminator Cycle Sequencing Kits are based
on the trusted Sanger Chain Termination method.

Format
The kits are delivered as a 2.5x concentrated ready-reaction
premix, fully optimized for a highly flexible chemistry, designed
for all kinds of sequencing applications, including de novo
sequencing and resequencing. The kits generate data with
uniform peak heights and optimized signal balance to produce
long, high-quality reads.

Two versions available
The BrilliantDye™ Terminator v1.1 Cycle Sequencing Kit is
designed for specialty applications, such as fragment analysis, that require optimal basecalling adjacent to the primer.

The BrilliantDye™ Terminator v3.1. Cycle Sequencing Kit is the method of choice for long reads.

Dye set
The kits are optimized to run with Dye Set E for BrilliantDye™
Terminator v1.1 and Dye Set Z for BrilliantDye™ Terminator v3.1.
Refer to your instrument manual how to calibrate the system
with this Dye Set.

Compatibility
The kits are compatible with ABI genetic analyzers of the 310,
3100, 3130, 3500, 3730 and SeqStudio series as well as the
Promega Spectrum system. No need for changing protocols,
workflows, settings and calibrations.

You also may need D-Pure™ DyeTerminator Cleanup,
NimaPOP™ polymers and running buffers, ExS-Pure™ enzymatic
PCR cleanup and ReCap capillary array regeneration system.

Protocol

v3.1